Distinguishbetweena purecultureandamixedculture.

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Exercise4: Purebacterialcolonies

Whenanagarplateis inoculated,whyis theloopsterilizedaftertheinitialinoculumis puton?

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Distinguishbetweena purecultureandamixedculture.

Definea bacterialcolony.Listfourcharacteristicsbywhichbacterialcoloniesmaybe distinguished.

Whyshoulda Petridishnotbeleftopenforanyextendedperiod?

Whydoesthestreakingmethod youusedto inoculateyourplatesresultin isolatedcolonies?

Exercise5: Pourplateandstreakingtechniquetoobtainpurecultures

Discusstherelativeconvenienceofpour-andstreak-platetechniquesinculturingclinicalspecimens.

Howdoyoudecidewhichcoloniesshouldbepickedfrom aplatecultureofamixedflora?

Whyisitnecessarytomakepuresubculturesoforganismsgrownfrom clinicalspecimens?

Whatkindsofclinicalspecimensmayyielda mixedflorainbacterialcultures?

Whenmorethanonecolonytypeappearsinpureculture,whatarethemostlikelysourcesof extraneouscontamination?

Exercise3: Primarymediaforisolationofmicroorganisms

1.Definea differentialmediumanddiscussitspurpose.

2.Definea selectivemediumanddescribeitsuses.

3.WhyisMacConkeyagarselectiveas wellasdifferential?

4.Whyisbloodagarusefulasaprimaryisolationmedium?

5.Whatis themajordifferencebetweenModifiedThayer-Martin(MTM)andchocolateagar?Whenwould youuseMTMratherthanchocolateagar?

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