How many colonies had recombinant DNA uptake?

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You are working in a research lab and one of your lucky lab mates Stephanie wins the lottery on th Show more You are working in a research lab and one of your lucky lab mates Stephanie wins the lottery on the way to lab and tells your advisor Dr. Jones she quits and is going to retire. Dr. Jones needs some new data from Stephanie urgently to put into her NSF grant proposal that is due the next day. Dr. Jones asks you to complete Stephanies experiments today! Dr. Jones explains to you that a ligation experiment attempted to insert Drosophila DNA into the LacZ gene of the plasmid vector pUC19. (Remember pUC19 has the LacZ gene and an ampr gene.) Stephanie then conducted a transformation experiment to insert products from the ligation experiment into cells of an ampsE.coli strain. Dr. Jones found a plate in the refrigerator labeled Drosophila Cloning Master with 24 white colonies. Dr.Jones is suspicious that the plate does not contain any antibiotics or X-Gal. Dr. Jones asks you to replica plate the colonies on to a plate containing ampicillin and X-Gal but unfortunately you can not find a plate with all of the additives but you did find a plate with ampicillin only and another plate with X-gal only. You realized that you can still get the data to Dr. Jones by tomorrow so you made two replica plates of the master plate; one replica is on the ampicillin only plate and one is on the X-Gal only plate. Colonies on the replica plates grew either white or blue. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Master Master Master X-Gal X-Gal X-Gal Ampicillin Ampicillin Ampicillin How many colonies had no DNA uptake? How many colonies had vector only DNA uptake? How many colonies had recombinant DNA uptake? Calculate the cloning efficiency. Round to two decimal places. (Answer in %) Show less